Determination of cyclophosphamide metabolites by gas chromatography and thermionic specific detection. Interindividual differences in hepatic biotransformation of cyclophosphamide in man in vitro.

نویسندگان

  • P J Sessink
  • W H Vaes
  • P H van den Broek
  • J Noordhoek
  • R P Bos
چکیده

Sensitive methods for the determination of the cyclophosphamide metabolites nornitrogen mustard, 4-ketocyclophosphamide and carboxyphosphamide are presented. After liquid-liquid extraction and derivatization, the metabolites are determined by gas chromatography and thermionic specific detection. The methods were used to study the in vitro biotransformation of cyclophosphamide with S-9 liver fractions of human donors. The results show large interindividual differences in the formation of nornitrogen mustard and carboxyphosphamide. 4-Ketocyclophosphamide was not detected.

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عنوان ژورنال:
  • Journal of chromatography. B, Biomedical applications

دوره 673 2  شماره 

صفحات  -

تاریخ انتشار 1995